ABIOTIC STRESSES INDUCE A THAUMATIN-LIKE PROTEIN IN MAIZE - CDNA ISOLATION AND SEQUENCE-ANALYSIS

被引：34

作者：

FRENDO, P ^{[1
]}

DIDIERJEAN, L ^{[1
]}

PASSELEGUE, E ^{[1
]}

BURKARD, G ^{[1
]}

机构：

[1] INST BIOL MOLEC PLANTES, 12 RUE GEN ZIMMER, F-67000 STRASBOURG, FRANCE

来源：

PLANT SCIENCE | 1992年 / 85卷 / 01期

关键词：

THAUMATIN-LIKE; MERCURIC CHLORIDE-INDUCED PROTEIN; UV-INDUCED PROTEIN; WOUND-INDUCED PROTEIN;

D O I：

10.1016/0168-9452(92)90094-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cDNA clone (CHEM4) coding for a stress-induced protein has been isolated from a mercuric chloride-treated maize (Zea mays L.) CDNA library by differential screening. Nucleotide sequence analysis of the 694-bp-cDNA insert predicts an open reading frame of 522 nucleotides encoding a mature protein of 149 amino acids and a signal peptide of 25 amino acid residues. The sequence of CHEM4-encoded protein shows 50 to 60% sequence identity with thaumatin, bifunctional alpha-amylase/trypsin inhibitor from maize and thaumatin-like (TL) proteins from dicotyledonous plants. Higher sequence identities are found to wheat PWIR2 and barley Hv-1b TL-proteins. CHEM4-mRNA is not only induced by mercuric chloride treatment but also by other stresses such as high salt concentration, high temperatures, UV light, wounding and polluted rainwater. CHEM4 genes appear to be highly inducible by UV light and mercuric chloride, moderately by wounding, salt stress, polluted rainwater and heat shock and very weakly by cooling.

引用

页码：61 / 69

页数：9

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