INFLUENCE OF PHOSPHOLIPIDIC MICROENVIRONMENT ON THE PERFORMANCE OF A POLYPYRROLE ENZYME ELECTRODE

被引：11

作者：

LECA, B ^{[1
]}

MORELIS, RM ^{[1
]}

COULET, PR ^{[1
]}

机构：

[1] UNIV LYON 1,ESCIL,GENIE ENZYMAT LAB,CNRS,URA 1535,43 BD 11 NOVEMBRE 1918,F-69622 VILLEURBANNE,FRANCE

来源：

TALANTA | 1994年 / 41卷 / 06期

关键词：

D O I：

10.1016/0039-9140(94)E0045-S

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Enzyme loaded phosphatidylcholine vesicles have been introduced as a new component in the design of sensing layers for the direct coating of an amperometric transducer. For this purpose, choline oxidase was chosen as a model enzyme, and the resulting vesicles were mixed with an amphiphilic pyrrole derivative [12-(pyrrol-1-yl)dodecyltriethylammonium tetrafluoroborate]. A minute amount of this mixture was deposited and dried on a platinum electrode, then electropolymerized. Compared to a similar preparation omitting phospholipid, the sensitivity of the enzyme electrode increased from 0.06 to 0.85 mA.1.mol-1. The response, obtained in less than 20 sec, was linearly related to the choline concentration within a broader range, extending from 2.5. 10(-7) to 3. 10(-5)M.

引用

页码：925 / 930

页数：6

共 22 条

[1]

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[2] DIFFUSION OF UNIVALENT IONS ACROSS LAMELLAE OF SWOLLEN PHOSPHOLIPIDS [J].