CRYOPRESERVATION OF TETRASELMIS-SUECICA CULTURED UNDER DIFFERENT NUTRIENTS REGIMES

被引：40

作者：

FENWICK, C

DAY, JG

机构：

[1] INST FRESHWATER ECOL,WINDERMERE LAB,CULTURE COLLECT ALGAE & PROTOZOA,AMBLESIDE LA22 0LP,CUMBRIA,ENGLAND

[2] UNIV DUNDEE,DEPT BIOL SCI,DUNDEE DD1 4HN,SCOTLAND

来源：

JOURNAL OF APPLIED PHYCOLOGY | 1992年 / 4卷 / 02期

关键词：

CRYOPRESERVATION; TETRASELMIS-SUECICA; PHOTOAUTOTROPHIC CULTURE; MIXOTROPHIC CULTURE; HETEROTROPHIC CULTURE;

D O I：

10.1007/BF02442458

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The commercially important member of the Prasinophyceae Tetraselmis suecica CCAP 66/4, which is widely used in the aquaculture industry, can be cryopreserved with levels of post-thaw viability in excess of 65%. The nutritional mode which the alga uses appears to be of secondary importance to the cryoprotectant and cooling protocol used, to maximise survival. Glycerol was the least cytotoxic and most protective of the cryoprotectants employed and a cooling rate of 5-degrees-C min-1 to -30-degrees-C with a 10-minute equilibration, followed by plunging into liquid nitrogen was found to give the highest levels of post-thaw viability.

引用

页码：105 / 109

页数：5