RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) PROFILING OF LEGIONELLA-PNEUMOPHILA

被引：15

作者：

SANDERY, M

COBLE, J

MCKERSIEDONNOLLEY, S

机构：

[1] Microbiology Laboratory, Division of Analytical Laboratories, New South Wales Health Department, Lidcombe, New South Wales

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1994年 / 19卷 / 04期

关键词：

D O I：

10.1111/j.1472-765X.1994.tb00939.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Random amplified polymorphic DNA (RAPD) profiling of Legionella pneumophila by PCR can be used to provide a simple and efficient comparison of clinical and environmental isolates. RAPD profiling is quicker and cheaper to perform than restriction fragment length polymorphism (RFLP) typing, eliminating the need for blotting, hybridization and detection. For some isolates, RAPD profiling is more discriminatory than RFLP typing, being able to distinguish between isolates with identical RFLP types.

引用

页码：184 / 187

页数：4

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