THE EXON TRAPPING ASSAY PARTLY DISCRIMINATES AGAINST ALTERNATIVELY SPLICED EXONS

被引：22

作者：

ANDREADIS, A

NISSON, PE

KOSIK, KS

WATKINS, PC

机构：

[1] BRIGHAM & WOMENS HOSP, DEPT MED, DIV NEUROL, CTR NEUROL DIS, BOSTON, MA 02115 USA

[2] GIBCO BRL LIFE TECHNOL INC, GAITHERSBURG, MD 20898 USA

来源：

NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 09期

关键词：

D O I：

10.1093/nar/21.9.2217

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cosmid containing eight exons of the gene coding for the microtubule-associated tau protein was subjected to the exon trapping assay. All the constitutive exons contained in the cosmid (4, 5, 7 and 9) were efficiently captured regardless of size. Of the four alternatively spliced exons, three (3, 4A and 8) were not isolated by the assay, but the behavior of exon 6 depended on the identity of its flanking exons.

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页码：2217 / 2221

页数：5

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