THE EXON TRAPPING ASSAY PARTLY DISCRIMINATES AGAINST ALTERNATIVELY SPLICED EXONS

被引：22

作者：

ANDREADIS, A

NISSON, PE

KOSIK, KS

WATKINS, PC

机构：

[1] BRIGHAM & WOMENS HOSP, DEPT MED, DIV NEUROL, CTR NEUROL DIS, BOSTON, MA 02115 USA

[2] GIBCO BRL LIFE TECHNOL INC, GAITHERSBURG, MD 20898 USA

来源：

NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 09期

关键词：

D O I：

10.1093/nar/21.9.2217

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cosmid containing eight exons of the gene coding for the microtubule-associated tau protein was subjected to the exon trapping assay. All the constitutive exons contained in the cosmid (4, 5, 7 and 9) were efficiently captured regardless of size. Of the four alternatively spliced exons, three (3, 4A and 8) were not isolated by the assay, but the behavior of exon 6 depended on the identity of its flanking exons.

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页码：2217 / 2221

页数：5

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共 28 条

[11] CLONING OF A BIG TAU-MICROTUBULE-ASSOCIATED PROTEIN CHARACTERISTIC OF THE PERIPHERAL NERVOUS-SYSTEM [J].

GOEDERT, M ;

SPILLANTINI, MG ;

CROWTHER, RA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (05) :1983-1987

[12] CLONING AND SEQUENCING OF THE CDNA-ENCODING AN ISOFORM OF MICROTUBULE-ASSOCIATED PROTEIN TAU CONTAINING 4 TANDEM REPEATS - DIFFERENTIAL EXPRESSION OF TAU PROTEIN MESSENGER-RNAS IN HUMAN-BRAIN [J].

GOEDERT, M ;

SPILLANTINI, MG ;

POTIER, MC ;

ULRICH, J ;

CROWTHER, RA .

EMBO JOURNAL, 1989, 8 (02) :393-399

[13] MULTIPLE ISOFORMS OF HUMAN MICROTUBULE-ASSOCIATED PROTEIN-TAU - SEQUENCES AND LOCALIZATION IN NEUROFIBRILLARY TANGLES OF ALZHEIMERS-DISEASE [J].

GOEDERT, M ;

SPILLANTINI, MG ;

JAKES, R ;

RUTHERFORD, D ;

CROWTHER, RA .

NEURON, 1989, 3 (04) :519-526

[14] TAU CONSISTS OF A SET OF PROTEINS WITH REPEATED C-TERMINAL MICROTUBULE-BINDING DOMAINS AND VARIABLE N-TERMINAL DOMAINS [J].

HIMMLER, A ;

DRECHSEL, D ;

KIRSCHNER, MW ;

MARTIN, DW .

MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (04) :1381-1388

[15] STRUCTURE OF THE BOVINE TAU-GENE - ALTERNATIVELY SPLICED TRANSCRIPTS GENERATE A PROTEIN FAMILY [J].

HIMMLER, A .

MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (04) :1389-1396

[16] A RAPID BOILING METHOD FOR THE PREPARATION OF BACTERIAL PLASMIDS [J].

HOLMES, DS ;

QUIGLEY, M .

ANALYTICAL BIOCHEMISTRY, 1981, 114 (01) :193-197

[17] DEVELOPMENTALLY REGULATED EXPRESSION OF SPECIFIC TAU SEQUENCES [J].

KOSIK, KS ;

ORECCHIO, LD ;

BAKALIS, S ;

NEVE, RL .

NEURON, 1989, 2 (04) :1389-1397

[18] OPTIMAL CONDITIONS FOR SUPERCOIL DNA SEQUENCING WITH THE ESCHERICHIA-COLI DNA-POLYMERASE-I LARGE FRAGMENT [J].

LIM, HM ;

PENE, JJ .

GENE ANALYSIS TECHNIQUES, 1988, 5 (02) :32-39

[19] USE OF RECOMBINANT DNA TECHNOLOGY TO PROGRAM EUKARYOTIC CELLS TO SYNTHESIZE RAT PROINSULIN - A RAPID EXPRESSION ASSAY FOR CLONED GENES [J].

LOMEDICO, PT .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (19) :5798-5802

[20] A CATALOG OF SPLICE JUNCTION SEQUENCES [J].

MOUNT, SM .

NUCLEIC ACIDS RESEARCH, 1982, 10 (02) :459-472