CARBOXYL-TERMINAL TRUNCATION OF APOLIPOPROTEINB-100 INHIBITS LIPOPROTEIN(A) PARTICLE FORMATION

被引：30

作者：

GABEL, B

YAO, ZM

MCLEOD, RS

YOUNG, SG

KOSCHINSKY, ML

机构：

[1] QUEENS UNIV,DEPT BIOCHEM,KINGSTON K7L 3N6,ON,CANADA

[2] UNIV ALBERTA,LIPID & LIPOPROT RES GRP,EDMONTON T6G 2S2,AB,CANADA

[3] UNIV ALBERTA,DEPT BIOCHEM,EDMONTON T6G 2S2,AB,CANADA

[4] UNIV CALIF SAN FRANCISCO,GLADSTONE INST CARDIOVASC DIS,CARDIOVASC RES INST,DEPT MED,SAN FRANCISCO,CA 94141

来源：

FEBS LETTERS | 1994年 / 350卷 / 01期

关键词：

LIPOPROTEIN(A); APOLIPOPROTEIN(A); APOLIPOPROTEINB-100; ASSEMBLY;

D O I：

10.1016/0014-5793(94)00737-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recombinant expression systems for both apo(a) and apoB were used to identify sequences in apoB which are required for Lp(a) formation. Incubation of a [S-35]Cys-labelled 17-kringle form of apo(a) with supernatants from rat hepatoma (McA-RH7777) cells expressing apoB-88, apoB-94 and apoB-100 resulted in covalent r-Lp(a) formation only with apoB-100. Additionally, apoB-86 present in the LDL of a hypobetalipoproteinemic subject did not associate with a 12-kringle form of recombinant apo(a) to form r-Lp(a) complexes. Our data suggest that sequences within the C-terminal 6% of apoB-100 are essential for Lp(a) assembly.

引用

页码：77 / 81

页数：5

共 21 条

[1] CYS4057 OF APOLIPOPROTEIN(A) IS ESSENTIAL FOR LIPOPROTEIN(A) ASSEMBLY [J].