IDENTIFICATION OF A MOUSE CDNA FRAGMENT WHOSE EXPRESSED POLYPEPTIDE REACTS WITH ANTI-RECA ANTIBODIES

被引:25
作者
ANGULO, JF
ROUER, E
BENAROUS, R
DEVORET, R
机构
[1] CNRS,ENZYMOL LAB,ETUD MUTAGENESE & CANCEROGENESE GRP,F-91198 GIF SUR YVETTE,FRANCE
[2] INST COCHIN GENET MOLEC,INSERM,U332,F-75014 PARIS,FRANCE
关键词
KIN17; PROTEIN; RECA PROTEIN; MOUSE EMBRYO; HOMEOBOX;
D O I
10.1016/0300-9084(91)90210-R
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have previously reported the in vivo detection of a mouse nuclear protein that cross-reacts with antibodies raised against E coli recA protein [1]. Here, we characterize monospecific anti-recA antibodies, their use for the immunological screening of a cDNA expression library and the isolation of a mouse cDNA fragment which codes for a polypeptide recognized by anti-recA antibodies. The cDNA fragment is 601 nucleotide long and was called KIN17(601). It contains an open reading frame coding for a 200 amino acid polypeptide. In kin17(200) polypeptide, there are amino acids identical to those that form one of the major antigenic determinants of recA protein. Kin17(200) polypeptide also displays a significant similarity with the helix 1 motif of several homeoproteins.
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页码:251 / 256
页数:6
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