TIMP-1 PROTEIN EXPRESSION IS STIMULATED BY IL-1-BETA AND IL-6 IN PRIMARY RAT HEPATOCYTES

被引：50

作者：

ROEB, E ^{[1
]}

GRAEVE, L ^{[1
]}

MULLBERG, J ^{[1
]}

MATERN, S ^{[1
]}

ROSEJOHN, S ^{[1
]}

机构：

[1] RHEIN WESTFAL TH AACHEN, INST BIOCHEM, D-52057 AACHEN, GERMANY

来源：

FEBS LETTERS | 1994年 / 349卷 / 01期

关键词：

TIMP-1; ANTIBODY; IL-6; IL-1; LIVER; HEPATOCYTE;

D O I：

10.1016/0014-5793(94)00636-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Degradation of extracellular matrix proteins is performed by metalloproteinases which are inhibited by tissue inhibitors of metalloproteinases (TIMP). We expressed the murine TIMP-1 protein in E. coli and prepared a polyclonal antiserum against the recombinant protein. Using this antiserum we studied the biosynthesis and glycosylation of murine TIMP-1 protein in COS-7 cells transfected with a TIMP-1 expression plasmid by metabolic labeling and indirect immunofluorescence studies. In primary rat hepatocytes we show for the first time that TIMP-1 protein expression is up-regulated upon stimulation with IL-1 beta and IL-6. Since TIMP-1 is induced during the acute phase reaction it could possibly be involved in the pathogenesis of liver fibrosis.

引用

页码：45 / 49

页数：5

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