STRUCTURAL-ANALYSIS OF PLANT RIBOSOMAL 5S RNAS - VISUALIZATION OF NOVEL TERTIARY INTERACTIONS BY CLEAVAGE OF LUPIN AND WHEAT 5SRRNAS WITH RIBONUCLEASE-H

被引：13

作者：

BARCISZEWSKA, MZ

LORENZ, S

ERDMANN, VA

BARCISZEWSKI, J

机构：

[1] POLISH ACAD SCI,INST BIOORGAN CHEM,NOSKOWSKIEGO 12,PL-61704 POZNAN,POLAND

[2] FREE UNIV BERLIN,INST BIOCHEM,W-1000 BERLIN 33,GERMANY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1087卷 / 01期

关键词：

(Lupin); (Wheat germ); Ribonuclease H; RNA structure; rRNA; 5S; Synthetic oligodeoxyribonucleotide;

D O I：

10.1016/0167-4781(90)90122-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A model for the tertiary structure of plant 5S rRNA, previously proposed by our laboratory (Joachimiak, A. et al. (1990) Int. J. Biol. Macromol., in press) was tested by specific cleavage of the plant 5S rRNA in the presence of synthetic oligodeoxynucleotides. The hexanucleotides used in this study were complementary to different portions of loops C, D and E, the nucleotides of which have recently been proposed to be involved in tertiary hydrogen bonds. The results obtained strongly support the interaction of loops C and D by nucelotides C34, C35, C36, A37 and G85, G86, G87, U88, respectively. Digestion pattern of loop E (domain gamma, nucleotides 66-110) suggests a possible different arrangement of this part of the plant 5S rRNA molecule, when compared with other eukaryotes. © 1990.

引用

页码：68 / 72

页数：5

共 20 条

[1] THE NUCLEOTIDE-SEQUENCE OF METHIONINE ELONGATOR TRANSFER-RNA FROM WHEAT-GERM [J].