INVOLVEMENT OF N-LINKED CARBOHYDRATE CHAINS OF PIG ZONA-PELLUCIDA IN SPERM-EGG BINDING

被引：79

作者：

YONEZAWA, N ^{[1
]}

AOKI, H ^{[1
]}

HATANAKA, Y ^{[1
]}

NAKANO, M ^{[1
]}

机构：

[1] CHIBA UNIV,GRAD SCH SCI & TECHNOL,CHIBA 260,JAPAN

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1995年 / 233卷 / 01期

关键词：

ZONA PELLUCIDA; N-LINKED CARBOHYDRATE CHAINS; N-GLYCANASE; SPERM-EGG BINDING;

D O I：

10.1111/j.1432-1033.1995.035_1.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The sperm receptor activity of pig zona pellucida has been previously shown to exist in one of the components, pig zona protein 3 alpha (PZP3 alpha), that can be purified after the removal of sialylated and/or sulfated N-acetylpoly(lactosamine) by digestion with endo-beta-galactosidase. In this study, we examined whether N-linked or O-linked carbohydrate chains are involved in the sperm receptor activity of pig zona pellucida. The elimination of N-linked carbohydrate chains from endo-beta-galactosidase-digested PZP3 alpha by digestion with N-glycanase markedly reduced its inhibitory effect on sperm-egg binding in an in vitro competition assay, whereas the elimination of O-linked carbohydrate chains by alkali treatment hardly reduced the inhibitory effect. These results indicate that N-linked carbohydrate chains of PZP3 alpha play a major role in mediating the sperm binding of zona pellucida in pig.

引用

页码：35 / 41

页数：7

共 27 条

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