TRANSFORMATION OF ASPERGILLUS-GIGANTEUS TO HYGROMYCIN-B RESISTANCE

被引：19

作者：

WNENDT, S ^{[1
]}

JACOBS, M ^{[1
]}

STAHL, U ^{[1
]}

机构：

[1] TECH UNIV BERLIN,FACHGEBIET MIKROBIOL & GENET,SEESTR 13,W-1000 BERLIN 65,GERMANY

来源：

CURRENT GENETICS | 1990年 / 17卷 / 01期

关键词：

A; giganteus; Dominant selection marker; Transformation system;

D O I：

10.1007/BF00313244

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A wild strain of A. giganteus was transformed to hygromycin B resistance using a bacterial resistance gene under the control of A. nidulans sequences. Stable transformants arose by heterogenous integration, mainly of tandem repeats of vector DNA at various sites in the host genome. Between 6 and 30 resistant colonies were obtained per μg DNA per 3×103 viable protoplasts. Vector DNA could be recovered by transformation of Escherichia coli with undigested genomic DNA from Aspergillus giganteus transformants. © 1990 Springer-Verlag.

引用

页码：21 / 24

页数：4

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