25-HYDROXYLATION OF VITAMIN-D3 IN RAT-LIVER - ROLES OF MITOCHONDRIAL AND MICROSOMAL CYTOCHROME-P-450

被引：16

作者：

DAHLBACK, H ^{[1
]}

WIKVALL, K ^{[1
]}

机构：

[1] UNIV UPPSALA, DEPT PHARMACEUT BIOCHEM, S-75123 UPPSALA, SWEDEN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1987年 / 142卷 / 03期

关键词：

D O I：

10.1016/0006-291X(87)91513-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A mitochondrial cytochrome P-450 fraction, which catalyzed 25-hydroxylation of vitamin D3 much more efficiently than intact mitochondria was isolated from livers of male and female rats. For comparison, a microsomal cytochrome P-450 fraction was isolated by the same procedures. The mitochondrial cytochrome P-450 from female rats catalyzed 25-hydroxylation as efficiently as the same material from male rats. The microsomal 25-hydroxylation was male specific. The 25-hydroxylase activity in intact mitochondria and the 25-hydroxyvitamin D3 concentration in serum were similar in male and female rats. There was no correlation between the 25-hydroxylase activity in microsomal cytochrome P-450 and the 25-hydroxyvitamin D3 concentration in serum.

引用

页码：999 / 1005

页数：7

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