VARIABILITY WITHIN THE CANDIDA-RUGOSA LIPASES FAMILY

被引：89

作者：

LOTTI, M

TRAMONTANO, A

LONGHI, S

FUSETTI, F

BROCCA, S

PIZZI, E

ALBERGHINA, L

机构：

[1] UNIV MILAN, DIPARTIMENTO FISIOL & BIOCHIM GEN, SEZ BIOCHIM COMPARATA, I-20133 MILAN, ITALY

[2] IRBM P ANGELETTI, ROME, ITALY

来源：

PROTEIN ENGINEERING | 1994年 / 7卷 / 04期

关键词：

FUNGI; GENE FAMILY; ISOZYMES; LIPASE; SPECIFICITY;

D O I：

10.1093/protein/7.4.531

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several fungi secrete lipase isozymes differing in biochemical properties and in some cases in substrate specificity. In the yeast Candida rugosa, a family of related genes encodes for multiple lipase proteins, highly homologous in sequence but partially different in the regions interacting with the substrate molecule. Analysis of these substitutions performed on the basis of multiple alignments and using a 3-D model of the enzyme, allows identification of a restricted number of amino acids possibly involved in substrate specificity of Candida lipases.

引用

页码：531 / 535

页数：5

共 28 条

[1] RECENT ADVANCES IN THE PURIFICATION, CHARACTERIZATION AND STRUCTURE DETERMINATION OF LIPASES [J].