TOXIC PRINCIPLE OF SELVA ANT VENOM IS A PORE-FORMING PROTEIN TRANSFORMER

被引：17

作者：

ARSENIEV, AS ^{[1
]}

PLUZHNIKOV, KA ^{[1
]}

NOLDE, DE ^{[1
]}

SOBOL, AG ^{[1
]}

TORGOV, MY ^{[1
]}

SUKHANOV, SV ^{[1
]}

GRISHIN, EV ^{[1
]}

机构：

[1] RUSSIAN ACAD SCI,SHEMYAKIN & OVCHINNIKOV INST BIOORGAN CHEM,MOSCOW 117871,RUSSIA

来源：

FEBS LETTERS | 1994年 / 347卷 / 2-3期

关键词：

ANT VENOM; ECTATOMIN; STRUCTURE; PORE-FORMING PROTEIN; ECTATOMMA TUBERCULATUM;

D O I：

10.1016/0014-5793(94)00518-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ectatomin (Ea) is a newly isolated main toxic component of Ectatomma tuberculatum ant venom. Structural and electrophysiological studies were performed with purified Ea. The protein consists of two homologous polypeptide chains (37 and 34 residues) and forms a four alpha-helix bundle in aqueous solution. On insertion into artificial bilayer membranes, two Ea molecules form an ion pore. Our results suggest that the 'inside-out' mechanism of pore formation requires a significant movement of Ea helical parts. The pore formation in the cell membrane might well explain the toxic activity of Ea, not excluding at the same time its intracellular activities.

引用

页码：112 / 116

页数：5

共 15 条

[11]

Schmidt J.O., 1986, P425

[12] COMPARATIVE ENZYMOLOGY OF VENOMS FROM STINGING HYMENOPTERA [J].

SCHMIDT, JO ;

BLUM, MS ;

OVERAL, WL .

TOXICON, 1986, 24 (09) :907-921

[13] CHARACTERIZATION OF CDNA CLONES FOR THE HUMAN-C-YES GENE [J].

SUKEGAWA, J ;

SEMBA, K ;

YAMANASHI, Y ;

NISHIZAWA, M ;

MIYAJIMA, N ;

YAMAMOTO, T ;

TOYOSHIMA, K .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (01) :41-47

[14] MECHANISMS OF MEMBRANE ASSEMBLY - GENERAL LESSONS FROM THE STUDY OF M13 COAT PROTEIN AND ESCHERICHIA-COLI LEADER PEPTIDASE [J].

WICKNER, W .

BIOCHEMISTRY, 1988, 27 (04) :1081-1086

[15] SEQUENTIAL RESONANCE ASSIGNMENTS AS A BASIS FOR DETERMINATION OF SPATIAL PROTEIN STRUCTURES BY HIGH-RESOLUTION PROTON NUCLEAR MAGNETIC-RESONANCE [J].

WUTHRICH, K ;

WIDER, G ;

WAGNER, G ;

BRAUN, W .

JOURNAL OF MOLECULAR BIOLOGY, 1982, 155 (03) :311-319

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