DETERMINATION OF POLYAMINES IN BIOLOGICAL-MATERIALS BY HPLC WITH 9-FLUORENYLMETHYL CHLOROFORMATE PRECOLUMN DERIVATIZATION

被引：21

作者：

HUHN, G ^{[1
]}

MATTUSCH, J ^{[1
]}

SCHULZ, H ^{[1
]}

机构：

[1] CTR ENVIRONM RES LEIPZIG HALLE, DEPT ANALYT CHEM, D-04301 LEIPZIG, GERMANY

来源：

FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 1995年 / 351卷 / 06期

关键词：

D O I：

10.1007/BF00322733

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Di- and polyamines have been determined in the femtomol range by liquid chromatography on a reversed-phase Cg column with a linear gradient of acetate buffer/acetonitrile as mobile phase and fluorimetric detection at 265/310 nm after simple derivatization with 9-fluorenylmethyl chloroformate (FMOC) within 3 min at pH 7.8 in presence of aspartic acid to remove excess reagent. The FMOC-polyamine-derivatives have been stable in a methanol-water-solution for at least 10 h. The method can be directly applied to crude plant extracts, and it is not interfered by carbohydrates and phenolics.

引用

页码：563 / 566

页数：4

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