EXPRESSION OF A HUMAN INSULIN-LIKE GROWTH FACTOR-II CDNA IN NIH-3T3 CELLS

被引:6
作者
BUERGISSER, DM [1 ]
ROTH, BV [1 ]
LUETHI, C [1 ]
GERBER, HP [1 ]
HONEGGER, A [1 ]
HUMBEL, RE [1 ]
机构
[1] RORER BIOTECHNOL INC,KING OF PRUSSIA,PA 19406
关键词
D O I
10.1016/0006-291X(90)91968-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Recombinant human insulin-like growth factor II (IGF II) was produced in NIH-3T3 cells transfected with a plasmid containing a construct encoding the signal peptide and the sequence of mature human IGF II. Successfully transfected clones secreted correctly processed recombinant human IGF II at rates of about 100 ng per 24 hours per 106 cells. The biological activity of the purified recombinant human IGF II exhibited similar potencies as standard human IGF II isolated from serum in radio-receptorassays as well as in thymidine incorporation assays. © 1990.
引用
收藏
页码:832 / 839
页数:8
相关论文
共 37 条
[1]   EXPRESSION OF A SYNTHETIC GENE ENCODING HUMAN INSULIN-LIKE GROWTH FACTOR-I IN CULTURED MOUSE FIBROBLASTS [J].
BAYNE, ML ;
CASCIERI, MA ;
KELDER, B ;
APPLEBAUM, J ;
CHICCHI, G ;
SHAPIRO, JA ;
PASLEAU, F ;
KOPCHICK, JJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (09) :2638-2642
[2]  
BAYNE ML, 1988, J BIOL CHEM, V263, P6233
[3]   OPTIMIZING THE EXPRESSION IN ESCHERICHIA-COLI OF A SYNTHETIC GENE ENCODING SOMATOMEDIN-C (IGF-I) [J].
BUELL, G ;
SCHULZ, MF ;
SELZER, G ;
CHOLLET, A ;
MOVVA, NR ;
SEMON, D ;
ESCANEZ, S ;
KAWASHIMA, E .
NUCLEIC ACIDS RESEARCH, 1985, 13 (06) :1923-1938
[4]   MUTANTS OF HUMAN INSULIN-LIKE GROWTH FACTOR-I WITH REDUCED AFFINITY FOR THE TYPE-1 INSULIN-LIKE GROWTH-FACTOR RECEPTOR [J].
CASCIERI, MA ;
CHICCHI, GG ;
APPLEBAUM, J ;
HAYES, NS ;
GREEN, BG ;
BAYNE, ML .
BIOCHEMISTRY, 1988, 27 (09) :3229-3233
[5]   INABILITY OF A MOUSE-CELL LINE TRANSFORMED TO PRODUCE BIOLOGICALLY-ACTIVE RECOMBINANT HUMAN INSULIN-LIKE GROWTH-FACTOR I (IGF-I) TO RESPOND TO EXOGENOUSLY ADDED IGF-I [J].
CASCIERI, MA ;
HAYES, NS ;
KELDER, B ;
KOPCHICK, JJ ;
CHICCHI, GG ;
SLATER, EE ;
BAYNE, ML .
ENDOCRINOLOGY, 1988, 122 (04) :1314-1320
[6]   ON THE NOMENCLATURE OF THE SOMATOMEDINS AND INSULIN-LIKE GROWTH-FACTORS [J].
DAUGHADAY, WH ;
HALL, K ;
SALMON, WD ;
VANDENBRANDE, JL ;
VANWYK, JJ .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1987, 65 (05) :1075-1076
[7]  
DAVIS LG, 1986, BASIC METHODS MOL BI, P93
[8]  
FROESCH ER, 1985, ANNU REV PHYSIOL, V47, P443
[9]   ULTRASTRUCTURAL-LOCALIZATION OF THE MANNOSE 6-PHOSPHATE RECEPTOR IN RAT-LIVER [J].
GEUZE, HJ ;
SLOT, JW ;
STROUS, GJAM ;
HASILIK, A ;
VONFIGURA, K .
JOURNAL OF CELL BIOLOGY, 1984, 98 (06) :2047-2054
[10]   DUAL AFFINITY FUSION APPROACH AND ITS USE TO EXPRESS RECOMBINANT HUMAN INSULIN-LIKE GROWTH FACTOR-II [J].
HAMMARBERG, B ;
NYGREN, PA ;
HOLMGREN, E ;
ELMBLAD, A ;
TALLY, M ;
HELLMAN, U ;
MOKS, T ;
UHLEN, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (12) :4367-4371