EXPRESSION OF A HUMAN INSULIN-LIKE GROWTH FACTOR-II CDNA IN NIH-3T3 CELLS

被引：6

作者：

BUERGISSER, DM ^{[1
]}

ROTH, BV ^{[1
]}

LUETHI, C ^{[1
]}

GERBER, HP ^{[1
]}

HONEGGER, A ^{[1
]}

HUMBEL, RE ^{[1
]}

机构：

[1] RORER BIOTECHNOL INC,KING OF PRUSSIA,PA 19406

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 169卷 / 03期

关键词：

D O I：

10.1016/0006-291X(90)91968-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recombinant human insulin-like growth factor II (IGF II) was produced in NIH-3T3 cells transfected with a plasmid containing a construct encoding the signal peptide and the sequence of mature human IGF II. Successfully transfected clones secreted correctly processed recombinant human IGF II at rates of about 100 ng per 24 hours per 106 cells. The biological activity of the purified recombinant human IGF II exhibited similar potencies as standard human IGF II isolated from serum in radio-receptorassays as well as in thymidine incorporation assays. © 1990.

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页码：832 / 839

页数：8

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