PROTEIN-STRUCTURE DETERMINATION WITH 3-DIMENSIONAL AND 4-DIMENSIONAL NMR-SPECTROSCOPY

Structural biology has made important contributions to the understanding of biological processes. In recent years an increasing amount of structural information has also been derived from NMR spectroscopic studies, often with special emphasis on dynamic aspects. The introduction of three‐ and four‐dimensional techniques has greatly simplified protein structure determination by NMR Spectroscopy, which has in fact become routine. In the past it was more of an art to interpret the complicated NOESY spectra of proteins, but the application of three‐dimensional techniques now makes the interpretation of protein spectra straightforward. In this review we discuss the most important multidimensional NMR techniques along with suitable applications. The emphasis is put less on the discussion of individual pulse sequences than on their application to the structure determination of proteins. Copyright © 1994 by VCH Verlagsgesellschaft mbH, Germany