FLUORIGENIC DERIVATIZATION OF OXAZEPAM - APPLICATION TO ITS DETERMINATION IN PHARMACEUTICALS

被引:2
作者
BERRUETA, LA
LAJUSTICIA, LF
GALLO, B
VICENTE, F
机构
[1] Department of Analytical Chemistry, Faculty of Sciences, University of the Basque Country, 48080, Bilbao
关键词
OXAZEPAM; 1,4-BENZODIAZEPINES; FLUOROMETRIC DERIVATIZATION; FLUOROMETRIC DETERMINATION IN PHARMACEUTICALS; FIA DETERMINATION IN PHARMACEUTICALS;
D O I
10.1080/00032719408006369
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Oxazepam, when heated in mixtures of acetic acid and methanol, undergoes a reaction giving an intense fluorescence at excitation and emission wavelengths of 364 and 469 nm, respectively. The best reaction conditions were a 7:25 methanol:acetic acid volume ratio, a temperature of 100 degrees C (examinated range 50-100 degrees C), and a reaction time of 5 minutes. A linear range from 0.025 to 50 mu g/ml with a limit of detection of 0.014 mu g/ml and a reproducibility within day of less than 5% were attained. A Flow Injection Analysis method was designed and a linear range from 0.1 to 100 mu g/ml with a limit of detection of 0.035 mu g/ml and a reproducibility within day of less than 5% were obtained. These methods were applied to the determination of oxazepam in five pharmaceutical formulations.
引用
收藏
页码:1295 / 1306
页数:12
相关论文
共 36 条
[1]  
AMAL H, 1973, J FAC PHARM ISTAMBUL, V9, P37
[2]  
BARGO ES, 1983, J ASSOC OFF ANA CHEM, V66, P864
[3]   BIOPHARMACOLOGICAL DATA AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF 1,4-BENZODIAZEPINES IN BIOLOGICAL-FLUIDS - A REVIEW [J].
BERRUETA, LA ;
GALLO, B ;
VICENTE, F .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1992, 10 (2-3) :109-136
[4]   ANALYSIS OF OXAZEPAM IN URINE USING SOLID-PHASE EXTRACTION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION BY POSTCOLUMN DERIVATIZATION [J].
BERRUETA, LA ;
GALLO, B ;
VICENTE, F .
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1993, 616 (02) :344-348
[5]  
BRAUN J, 1968, CAN J PHARM SCI, V3, P65
[6]  
CAILLE G, 1973, CAN J PHARM SCI, V8, P42
[7]  
CAILLE G, 1970, CAN J PHARM SCI, V5, P78
[8]   DETERMINATION OF SOME 1,4-BENZODIAZEPINES AND THEIR METABOLITES IN BODY-FLUIDS - REVIEW [J].
CLIFFORD, JM ;
SMYTH, WF .
ANALYST, 1974, 99 (1178) :241-272
[9]   ANALYSIS OF BENZODIAZEPINES .2. HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY FLUORESCENCE DETECTION AFTER MOLECULAR REARRANGEMENT TO ACRIDANONES [J].
DEGIOVANNI, N ;
CHIAROTTI, M .
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1988, 428 (02) :321-329
[10]   SIMULTANEOUS DETERMINATION OF BENZODIAZEPINES BY ULTRAVIOLET - VISIBLE SPECTROPHOTOMETRY IN MICELLAR MEDIA [J].
DELAGUARDIA, M ;
GALDU, MV ;
MONZO, J ;
SALVADOR, A .
ANALYST, 1989, 114 (04) :509-512