ASSIGNMENT OF 112 MICROSATELLITE MARKERS TO 23 CHROMOSOME-11 SUBREGIONS DELINEATED BY SOMATIC HYBRIDS - COMPARISON WITH THE GENETIC-MAP

被引：31

作者：

COUILLIN, P

LEGUERN, E

VIGNAL, A

FIZAMES, C

RAVISE, N

DELPORTES, D

REGUIGNE, I

ROSIER, MF

JUNIEN, C

VANHEYNINGEN, V

WEISSENBACH, J

机构：

[1] HOP LA PITIE SALPETRIERE,INSERM,U289,F-75651 PARIS 13,FRANCE

[2] GENETHON,F-91000 EVRY,FRANCE

[3] CNRS,UPR 420,F-94801 VILLEJUIF,FRANCE

[4] INSERM,U73,F-75016 PARIS,FRANCE

[5] WESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLAND

来源：

GENOMICS | 1994年 / 21卷 / 02期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1006/geno.1994.1280

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Using a panel of 25 somatic cell hybrids, we have regionally localized 112 microsatellite markers generated by Genethon and assigned to chromosome II. A genetic map of 74 of them was produced using linkage analysis of the eight largest CEPH (Centre d'Etude du Polymorphisme Humain) families. They could be ordered on chromosome 11 with an average distance of 2.1 cM. The tight correlation observed between the genetic order and the physical assignment of these microsatellites reinforces the genetic map data. These newly localized markers identified by the PCR method using a standardized protocol represent useful tools for mapping YAC clones and establishing YAC contigs and for studying genetic diseases or cancers associated with specific genes and/or germinal/somatic rearrangements of chromosome II. (C) 1994 Academic Press, Inc.

引用

页码：379 / 387

页数：9

共 60 条

[1] REDUCTION TO HOMOZYGOSITY OF GENES ON CHROMOSOME-11 IN HUMAN-BREAST NEOPLASIA [J].