THE MITOCHONDRIAL PYRUVATE-DEHYDROGENASE COMPLEX - NUCLEOTIDE AND DEDUCED AMINO-ACID-SEQUENCES OF A CDNA-ENCODING THE ARABIDOPSIS-THALIANA E1-ALPHA-SUBUNIT

被引：33

作者：

LUETHY, MH ^{[1
]}

MIERNYK, JA ^{[1
]}

RANDALL, DD ^{[1
]}

机构：

[1] UNIV MISSOURI, DEPT BIOCHEM, COLUMBIA, MO 65211 USA

来源：

GENE | 1995年 / 164卷 / 02期

基金：

美国国家科学基金会;

关键词：

CARBON METABOLISM; PHOSPHORYLATION; PLANT;

D O I：

10.1016/0378-1119(95)00465-I

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A cDNA encoding the E1 alpha subunit of the Arabidopsis thaliana (At) mitochondrial (mt) pyruvate dehydrogenase complex (PDC) was sequenced. The 1435-bp cDNA consists of a 1167-bp open reading frame encoding a 43.0-kDa polypeptide of 389 amino acids (aa) (pI 7.1). The plant E1 alpha subunit has 47-51% aa sequence identity with other eukaryotic sequences. Among the regions that are highly conserved are the aa surrounding phosphorylation sites 1 and 2 of the mammalian sequence, including the conserved Ser(292) residue of At at site 1. An essential active site residue, Cys(62) Of the bovine subunit, is also conserved. A 32-aa presumptive mt targeting sequence is present at the N terminus.

引用

页码：251 / 254

页数：4