RADIOIMMUNOASSAY OF INSULIN-LIKE GROWTH-FACTORS IN CYST FLUID OF CENTRAL-NERVOUS-SYSTEM TUMORS

被引:30
作者
GLICK, RP
UNTERMAN, TG
HOLLIS, R
机构
[1] UNIV ILLINOIS, COLL MED, DEPT NEUROSURG, CHICAGO, IL 60680 USA
[2] UNIV ILLINOIS, COLL MED, DEPT MED, CHICAGO, IL 60680 USA
[3] UNIV ILLINOIS, COLL MED, DEPT MED RES, CHICAGO, IL 60680 USA
[4] VET ADM W SIDE MED CTR, CHICAGO, IL 60680 USA
关键词
INSULIN-LIKE GROWTH FACTOR; GROWTH FACTOR; SOMATOMEDIN; TUMOR; BINDING PROTEIN; RADIOIMMUNOASSAY; CYST;
D O I
10.3171/jns.1991.74.6.0972
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Tumor cells are characterized by abnormalities in growth and metabolism, including the autocrine secretion of certain growth factors. The authors have previously shown the presence of insulin-like growth factor receptors in tumors of the central nervous system (CNS) and in this study examine whether CNS tumors are capable of autocrine secretion of insulin-like growth factors in situ. To investigate the production of insulin-like growth factors I and II by CNS tumors, the authors have developed specific radioimmunoassays for these growth factors. In situ production of insulin-like growth factors was studied by immunoassay of CNS tumor cyst fluid aspirated at the time of surgery from 12 cystic tumors: seven primary brain tumors, four metastatic tumors, and one spinal schwannoma. For immunoassay, cyst fluid was treated overnight with acetic acid, then insulin-like growth factors were separated from binding proteins by a refined solid-phase technique, then dried and reconstituted in immunoassay buffer. Normal human serum and cerebrospinal fluid served as controls. Insulin-like growth factor I was detected in all 12 tumors studied. In contrast, insulin-like growth factor II was detected only in three low-grade astrocytomas, the spinal schwannoma (which had the highest insulin-like growth factor II level of all tumors studied), and three metastatic lung cancers. These results suggest that CNS tumors may be capable of autocrine production of insulin-like growth factors in situ. Furthermore, there appears to be a difference in the type of insulin-like growth factors produced by different types of CNS tumors. Preferential production of insulin-like growth factors may be an important marker of tumor differentiation and useful as a diagnostic tool.
引用
收藏
页码:972 / 978
页数:7
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