PRODUCTION OF HIGH GLUCOSE SYRUPS USING GLUCOAMYLASE IMMOBILIZED ON ALKYLAMINE DERIVATIVES OF TITANIUM(IV)-ACTIVATED POROUS SILICA - KINETICS AND OPERATIONAL STABILITY

Glucoamylase (EC 3. 2. 1. 3) was immobilized on controlled pore silica by a new covalent method of enzyme immobilization on inorganic supports. A 30 wt% starch hydrolysate solution with 40 DE (DE is the dextrose equivalent) was used as substrate. The kinetic constants of the immobilized enzyme (IME) and of the soluble enzyme were determined. The influences of pH and temperature on activity were studied, a pH of 4. 5 and a temperature of 60 degree C being the optimum values for both forms of the enzyme. The operational stability of the IME was studied at various temperatures and the optimal temperature in terms of productivity was found to be 50 degree C, the half-life of the IME being then 38 days.