PERSISTENT EFFECT OF TGF-BETA-1 ON EXTRACELLULAR-MATRIX GENE-EXPRESSION IN HUMAN DERMAL FIBROBLASTS

被引：41

作者：

ISHIKAWA, O

YAMAKAGE, A

LEROY, EC

TROJANOWSKA, M

机构：

[1] Division of Rheumatology and Immunology, Department of Medicine Medical University of South Carolina, Charleston, SC

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 169卷 / 01期

关键词：

D O I：

10.1016/0006-291X(90)91458-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

TGF-β, a potent inducer of the extracellular matrix, is also known to stimulate its own synthesis. In this report we have analyzed long term effects of TGF-β1 on its own expression and on the expression of extracellular matrix genes. We demonstrated that 24 hours of incubation of human dermal fibroblasts with TGF-β1 (1 ng/ml) under serum free conditions resulted in an elevated expression of TGF-β1, collagen α2(I) and fibronectin mRNAs that persisted at least 96 hours after removal of TGF-β1. These data suggest the possibility of persistent in vivo activation of target cells following exposure to TGF-β1. © 1990.

引用

页码：232 / 238

页数：7

共 24 条

[1] CHEIFETZ S, 1986, J BIOL CHEM, V261, P9972
[2] CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[3] CROMBRUGGHE B, 1990, RHEUM DIS CLIN, V16
[4] FROLIK CA, 1984, J BIOL CHEM, V259, P995
[5] PURIFICATION AND INITIAL CHARACTERIZATION OF A TYPE-BETA TRANSFORMING GROWTH-FACTOR FROM HUMAN-PLACENTA
FROLIK, CA
DART, LL
MEYERS, CA
SMITH, DM
SPORN, MB
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (12): : 3676 - 3680
[6] ISHIKAWA O, UNPUB J CELL PHYSL
[7] KAHALEH MBB, 1988, CLIN IMMUNOL IMMUPAT
[8] KIM SJ, 1989, J BIOL CHEM, V264, P7041
[9] KULOZIK M, 1989, J INVEST DERMATOL, V92, P465
[10] LE JM, 1987, LAB INVEST, V56, P234

← 1 2 3 →