PHOTOCHEMICAL IMMOBILIZATION OF HEPARIN, DERMATAN SULFATE, DEXTRAN SULFATE AND ENDOTHELIAL-CELL SURFACE HEPARAN-SULFATE ONTO CELLULOSE MEMBRANES FOR THE PREPARATION OF ATHROMBOGENIC AND ANTITHROMBOGENIC POLYMERS

被引:45
作者
ERDTMANN, M
KELLER, R
BAUMANN, H
机构
[1] RHEIN WESTFAL TH AACHEN,W-5100 AACHEN,GERMANY
[2] KLINIKUM RWTH AACHEN,AACHEN,GERMANY
关键词
IMMOBILIZATION; PHOTOSENSITIVE HETEROBIFUNCTIONAL CROSS-LINKING REAGENT; HEPARIN; ES-HS; PLATELET ADHESION TEST;
D O I
10.1016/0142-9612(94)90089-2
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Heparin (HE), dextran sulphate (DX) of molecular weight 40000 and 500000, dermatan sulphate (DS) and endothelial cell surface heparan sulphate (ES-HS) were immobilized covalently onto cellulose membranes (Visking(R) dialysis tubes) using the photochemical heterobifunctional reagent 4-azido-1-fluoro-2-nitrobenzene (AFNB); 120 pmol HE/cm(2) and 40 pmol DS/cm(2), 3.4 pmol DX 500 000/cm(2), 50 pmol DX 40 000/cm(2) and 3.6 pmol ES-HS/cm(2) were immobilized. The platelet adhesion of the modified membranes was measured in a modified Baumgartner perfusion chamber with citrated human blood at a defined shear rate. Membranes modified with DX 40 000 and DX 500 000 showed 80% and 30% platelet adhesion, respectively, heparinized and DS coated membranes showed 50% and 60% platelet adhesion, respectively, compared with a subendothelial matrix (100% platelet adhesion). ES-HS modified membranes showed no platelet adhesion.
引用
收藏
页码:1043 / 1048
页数:6
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