ISOLATION AND IDENTIFICATION OF A PHEROMONOTROPIC NEUROPEPTIDE FROM THE BRAIN-SUBESOPHAGEAL GANGLION COMPLEX OF LYMANTRIA-DISPAR - A NEW MEMBER OF THE PBAN FAMILY

被引：82

作者：

MASLER, EP ^{[1
]}

RAINA, AK ^{[1
]}

WAGNER, RM ^{[1
]}

KOCHANSKY, JP ^{[1
]}

机构：

[1] USDA ARS,INST LIVESTOCK & POULTRY SCI,LIVESTOCK INSECTS LAB,BELTSVILLE,MD 20705

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1994年 / 24卷 / 08期

关键词：

AMINO ACID SEQUENCE; GYPSY MOTH; LYMANTRIA DISPAR; NEUROPEPTIDE ISOLATION; PBAN SEQUENCE; HOMOLOGY; PHEROMONOTROPIC PEPTIDES;

D O I：

10.1016/0965-1748(94)90111-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A pheromonotropic peptide was isolated from brain-suboesophageal ganglion complexes of the adult female gypsy moth, Lymantria dispar, using a 5-step HPLC purification protocol and an in vivo bioassay in Helicoverpa zea. The intact peptide was sequenced by automated Edman degradation. The L. dispar pheromone biosynthesis activating neuropeptide (Lyd-PBAN) is a C-terminally amidated 33-amino acid peptide with a molecular weight of 3881. The peptide was synthesized using Fmoc procedures. Lyd-PBAN has sequence homology with Hez-PBAN (81.8%) and Bom-PBAN-I (66.7%). All three PBANs share the C-terminal hexapeptide sequence, Tyr-Phe-Ser-Pro-Arg-Leu-NH,. Tn addition, the C-terminal pentapeptide sequences of Pseudaletia pheromonotropin (Pss-PT), Bombyx diapause hormone (Bom-DH), the locustamyotropins (Lom-MT) and leucopyrokinin (Lem-PK) are identical or have a high degree of homology to the C-terminus of PBANs.

引用

页码：829 / 836

页数：8

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