MOLECULAR-CLONING OF A HUMAN POLYPEPTIDE RELATED TO YEAST SDS22, A REGULATOR OF PROTEIN PHOSPHATASE-1

被引:37
作者
RENOUF, S
BEULLENS, M
WERA, S
VAN EYNDE, A
SIKELA, J
STALMANS, W
BOLLEN, M
机构
[1] KATHOLIEKE UNIV LEUVEN, FAC GENEESKUNDE,AFDELING BIOCHEM, B-3000 LOUVAIN, BELGIUM
[2] UNIV COLORADO, HLTH SCI CTR,DEPT PHARMACOL, DENVER, CO 80262 USA
关键词
CELL CYCLE; DEPHOSPHORYLATION; MITOSIS; PROTEIN PHOSPHATASE;
D O I
10.1016/0014-5793(95)01180-M
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
sds22 is a regulatory polypeptide of protein phosphatase-1 that is required for the completion of mitosis in both fission and budding yeast, We report here the cDNA cloning of a human polypeptide that is 46% identical to yeast sds22. The human homolog of sds22 consists of 360 residues, has a calculated molecular mass of 41.6 kDa and shows a tandem array of 11 leucine-rich repeat structures of 22 residues, Northern analysis revealed a major transcript of 1.39 kb in all 8 investigated human tissues. sds22 was detected by western analysis in both the cytoplasm and the nucleus of rat liver cells as a polypeptide of 44 kDa.
引用
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页码:75 / 78
页数:4
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