MOLECULAR-CLONING OF A HUMAN POLYPEPTIDE RELATED TO YEAST SDS22, A REGULATOR OF PROTEIN PHOSPHATASE-1

被引：37

作者：

RENOUF, S

BEULLENS, M

WERA, S

VAN EYNDE, A

SIKELA, J

STALMANS, W

BOLLEN, M

机构：

[1] KATHOLIEKE UNIV LEUVEN, FAC GENEESKUNDE,AFDELING BIOCHEM, B-3000 LOUVAIN, BELGIUM

[2] UNIV COLORADO, HLTH SCI CTR,DEPT PHARMACOL, DENVER, CO 80262 USA

来源：

FEBS LETTERS | 1995年 / 375卷 / 1-2期

关键词：

CELL CYCLE; DEPHOSPHORYLATION; MITOSIS; PROTEIN PHOSPHATASE;

D O I：

10.1016/0014-5793(95)01180-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

sds22 is a regulatory polypeptide of protein phosphatase-1 that is required for the completion of mitosis in both fission and budding yeast, We report here the cDNA cloning of a human polypeptide that is 46% identical to yeast sds22. The human homolog of sds22 consists of 360 residues, has a calculated molecular mass of 41.6 kDa and shows a tandem array of 11 leucine-rich repeat structures of 22 residues, Northern analysis revealed a major transcript of 1.39 kb in all 8 investigated human tissues. sds22 was detected by western analysis in both the cytoplasm and the nucleus of rat liver cells as a polypeptide of 44 kDa.

引用

页码：75 / 78

页数：4

共 17 条

[11] S-POMBE GENE SDS22+ ESSENTIAL FOR A MIDMITOTIC TRANSITION ENCODES A LEUCINE-RICH REPEAT PROTEIN THAT POSITIVELY MODULATES PROTEIN PHOSPHATASE-1 [J].