DIFFERENTIAL REGULATION OF PHOSPHOLIPASE-A2 BY CYTOKINES INHIBITING BONE-FORMATION AND MINERALIZATION

被引:5
作者
ELLIES, LG [1 ]
GUPTA, AK [1 ]
AUBIN, JE [1 ]
机构
[1] UNIV TORONTO, MRC, PERIODONTAL PHYSIOL GRP, TORONTO M5S 1A8, ONTARIO, CANADA
关键词
D O I
10.1016/0006-291X(92)91337-P
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Treatment of fetal rat calvarial cells with interleukin-lα, tumor necrosis factor-α, transforming growth factor-β1, or group II phospholipase A2 inhibits the number of bone nodules formed in long-term cultures. These same mediators also inhibit the mineralization of fully developed bone nodules in a time and dose-dependent fashion. The pro-inflammatory cytokines interleukin-α and tumor necrosis factor-α cause a dose-dependent induction of rat calvarial cell phospholipase A2-II mRNA levels, suggesting that their effects on bone formation may be mediated indirectly by activation of this enzyme. In contrast, transforming growth factor-β1, which has more potent effects on bone formation than interleukin-α or tumor necrosis factor-α, supresses basal levels of phospholipase A2-II mRNA, indicating a different mechanism of action for this cytokine. © 1992.
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页码:1047 / 1053
页数:7
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