E1A AND RAS ONCOGENES SYNERGISTICALLY ENHANCE RECOMBINANT PROTEIN-PRODUCTION UNDER CONTROL OF THE CYTOMEGALOVIRUS PROMOTER IN BHK-21-CELLS

被引：18

作者：

SHIRAHATA, S

WATANABE, J

TERUYA, K

YANO, T

OSADA, K

OHASHI, H

TACHIBANA, H

KIM, EH

MURAKAMI, H

机构：

[1] KIRIN BREWERY CO LTD, PHARMACEUT LAB, MAEBASHI, GUMMA 371, JAPAN

[2] KOREA UNIV, DEPT BIOTECHNOL, CHOCHIWON 339800, SOUTH KOREA

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1995年 / 59卷 / 02期

关键词：

D O I：

10.1271/bbb.59.345

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The amplified ras oncogene greatly enhanced the production of recombinant human interleukin-6 (hIL-6) under control of the cytomegalovirus immediate early promoter (CMV promoter) in BHK-21 cells. When the adenovirus E1A oncogene was further transfected into the above mentioned ras-amplified hIL-6 hyperproducing BHK cells, the transfectants had about 10 times higher productivity than non-transfectants, However, the E1A gene alone did not enhance productivity. These results implicate a vas and E1A synergistic ability that acts to enhance hIL-6 production.

引用

页码：345 / 347

页数：3

共 23 条

[1] P21RAS CONTRIBUTES TO HIV-1 ACTIVATION IN T-CELLS [J].