SOLID-PHASE NONISOTOPIC LABELING OF OLIGODEOXYNUCLEOTIDES USING 5'-PROTECTED AMINOALKYL PHOSPHORAMIDITES - APPLICATION TO THE SPECIFIC DETECTION OF HUMAN PAPILLOMA-VIRUS DNA

被引：8

作者：

DEVOS, MJ ^{[1
]}

CRAVADOR, A ^{[1
]}

LENDERS, JP ^{[1
]}

HOUARD, S ^{[1
]}

BOLLEN, A ^{[1
]}

机构：

[1] UNIV BRUSSELS,SERV GENET APPL,RUE IND 24,B-1400 NIVELLES,BELGIUM

来源：

NUCLEOSIDES & NUCLEOTIDES | 1990年 / 9卷 / 02期

关键词：

D O I：

10.1080/07328319008045137

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phosphoramidites of thymidine or 2'-deoxyinosine, modified in 5' by the addition of an aminoalkylcarbamate function, were prepared The derivatized nucleotides can be used in automatic DNA synthesis to tag any oligodeoxynucleotide chain and provide a convenient reactive group for labelling with non radioactive reporters. As an example of application, we show the specific detection of Human Papilloma Virus DNA using a biotinlabelled 29-mer oligodeoxynucleotide entirely prepared on solid support. © 1990, Taylor & Francis Group, LLC. All rights reserved.

引用

页码：259 / 273

页数：15

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