MICROENCAPSULATION OF LACTOCOCCUS-LACTIS SUBSP CREMORIS

被引:38
作者
LARISCH, BC [1 ]
PONCELET, D [1 ]
CHAMPAGNE, CP [1 ]
NEUFELD, RJ [1 ]
机构
[1] MCGILL UNIV,DEPT CHEM ENGN,3480 UNIV ST,MONTREAL H3A 2A7,QUEBEC,CANADA
关键词
D O I
10.3109/02652049409040450
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Lactococcus lactis subsp. cremoris was microencapsulated within alginate/poly-L-lysine (alg/PLL), nylon or crosslinked polyethyleneimine (PEI) membranes. Toxic effects were observed with solvents and reagents used in nylon and PEI membrane formation. Alg/PLL encapsulation resulted in viable and active cell preparations which acidified milk at a rate proportional to the cell concentration, but at rates less than that of free cell preparations. At 4 X 10(8) colony-forming units (cfu/ml milk), encapsulated cells took 17 per cent longer than free lactococci to reduce the pH of milk to 5.5. Similar activities of free and microencapsulated cells may be attained at higher cell concentrations (10(9) cfu/ml milk). The rate of lactic acid production was approximately 2 mmol/h at an encapsulated cell concentration of 4 X 10(8) cfu/ml.
引用
收藏
页码:189 / 195
页数:7
相关论文
共 21 条
[1]  
Brink L.E.S., Tramper J., Optimization of organic solvent in multiphase biocatalysis, Biotechnology and Bioengineering, 27, pp. 1258-1269, (1985)
[2]  
Champagne C.P., Inhibition of psychrotrophic bacteria in raw milk by immobilized lactic acid bacteria, Biotechnology Letters, 12, pp. 771-776, (1990)
[3]  
Champagne C.P., COTE C.B., Cream fermentation by immobilized lactic acid bacteria, Biotechnology Letters, 9, pp. 329-332, (1987)
[4]  
Champagne C.P., COTE C.B., Goulet J., [Fermentation of lacto-serum by immobilized cells of, Lactobacillus helveticus]. Canadian Institute of Food Science and Technology Journal, 21, pp. 403-407, (1988)
[5]  
Champagne C.P., COTE C.B., Goulet J., Whey fermentation by immobilized cells of, Propionibacterium shermanii. Journal of Applied Bacteriology, 66, pp. 175-184, (1989)
[6]  
Champagne C.P., Gaudy C., Poncelet D., Neufeld R.J., Lactococcus lactis release from calcium alginate beads, Applied and Environmental Microbiology, 58, pp. 1429-1434, (1992)
[7]  
Champagne C.P., Girard F., Morin N., b, Bacteriophage development in an immobilized lactic acid bacteria system, Biotechnology Letters, 10, pp. 463-468, (1988)
[8]  
Cogan T.M., [The lactic mesophilic yeasts. A review], Le Lait, 60, pp. 397-425, (1980)
[9]  
Hulst A.C., Tramper J., Brodelius P., Eijkenboom L.J.C., Luyben C.A.M., Immobilized plant cells: respiration and oxygen transfer, Journal of Chemical Technology and Biotechnology, 35, pp. 198-204, (1985)
[10]  
Kearney L., Upton M., McLaughlin A., Meat fermentations with immobilized lactic acid bacteria, Applied Microbiology and Biotechnology, 33, pp. 648-651, (1990)