MICROENCAPSULATION OF LACTOCOCCUS-LACTIS SUBSP CREMORIS

被引：38

作者：

LARISCH, BC ^{[1
]}

PONCELET, D ^{[1
]}

CHAMPAGNE, CP ^{[1
]}

NEUFELD, RJ ^{[1
]}

机构：

[1] MCGILL UNIV,DEPT CHEM ENGN,3480 UNIV ST,MONTREAL H3A 2A7,QUEBEC,CANADA

来源：

JOURNAL OF MICROENCAPSULATION | 1994年 / 11卷 / 02期

关键词：

D O I：

10.3109/02652049409040450

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Lactococcus lactis subsp. cremoris was microencapsulated within alginate/poly-L-lysine (alg/PLL), nylon or crosslinked polyethyleneimine (PEI) membranes. Toxic effects were observed with solvents and reagents used in nylon and PEI membrane formation. Alg/PLL encapsulation resulted in viable and active cell preparations which acidified milk at a rate proportional to the cell concentration, but at rates less than that of free cell preparations. At 4 X 10(8) colony-forming units (cfu/ml milk), encapsulated cells took 17 per cent longer than free lactococci to reduce the pH of milk to 5.5. Similar activities of free and microencapsulated cells may be attained at higher cell concentrations (10(9) cfu/ml milk). The rate of lactic acid production was approximately 2 mmol/h at an encapsulated cell concentration of 4 X 10(8) cfu/ml.

引用

页码：189 / 195

页数：7

共 21 条

[11]

Kierstan M., Bucke C., The immobilization of microbial cells, subcellular organelles, and enzymes in calcium alginate gels, Biotechnology and Bioengineering, 19, pp. 387-397, (1977)

[12]

Kim H.S., Kamara B.J., Good I.C., Enders G.L., Method for the preparation of stable microencapsulated lactic acid bacteria, Journal of Industrial Microbiology, 3, pp. 253-257, (1988)

[13]

Kolot F.B., Immobilized Microbial Systems: Principles, Techniques, and Industrial Applications, (1988)

[14]

Lencki R.W.J., Neufeld R.J., Spinney T., Method of producing microspheres, (1989)

[15]

Linko P., Immobilized lactic acid bacteria, in Enzymes and Immobilized Cells in Biotechnology, pp. 25-36, (1985)

[16]

Lucas S., Reyrolle J., [Study on a series of lactic mesophilic fermentations. Balance of cells during the first stage of leaven production], Lait, 69, pp. 121-130, (1989)

[17]

Marx J.L., A Revolution in Biotechnology, (1989)

[18]

Morin N., Bernier-Cardou M., Champagne C.P., Production of concentrated Lactococcus lactis cultures in calcium alginate beads, Applied and Environmental Microbiology, 58, pp. 545-550, (1992)

[19]

Neufeld R.J., Peleg Y., Roken J.S., Pines O., Goldberg I., L-malic acid formation by immobilized Saccharomyces cerevidiae amplified for fumarase, Enzyme and Microbial Technology, 13, pp. 991-996, (1991)

[20]

Stadhouders J., Hassing F., Description of a method for determining the activity of cheese starters, International Dairy Federation document, 129, pp. 9-11, (1980)

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