CHARACTERIZATION OF A HTLV-I-INFECTED CELL-LINE DERIVED FROM A PATIENT WITH ADULT T-CELL LEUKEMIA WITH STABLE COEXPRESSION OF CD4 AND CD8

被引:14
作者
ROWE, T
DEZZUTTI, C
GUENTHNER, PC
LAM, L
HODGE, T
LAIRMORE, MD
LAL, RB
FOLKS, TM
机构
[1] CTR DIS CONTROL & PREVENT,DIV VIRAL & RICKETTSIAL DIS,RETROVIRUS DIS BRANCH,ATLANTA,GA 30333
[2] CTR DIS CONTROL & PREVENT,NCID,DIV HIV AIDS,IMMUNOL BRANCH,ATLANTA,GA 30333
[3] OHIO STATE UNIV,CTR RETROVIRUS RES,COLUMBUS,OH 43210
[4] OHIO STATE UNIV,DEPT VET BIOSCI,COLUMBUS,OH 43210
关键词
ATL; CELL LINE; DUAL CD+ CD8(+) POSITIVE;
D O I
10.1016/0145-2126(95)00030-R
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
A long-term T-cell line, termed SP+, was developed from a human T-cell leukemia virus type I (HTLV-I)-infected patient with adult T-cell leukemia that is dependent on exogenous IL-2 for growth. The SP+ expresses a full complimentation of HTLV-I-specific viral proteins, and contains replication competent viral particles. Restriction enzyme digestion followed by Southern blot analysis demonstrated the presence of a single integrated proviral copy and limiting dilution analysis confirmed the clonality of the cell line. Interestingly, phenotypically, the SP+ cell line is CD2(+), CD3(+) and coexpresses CD4 and CD8, yet lacks TCR alpha beta and TCR tau delta expression. Further ontogenetic characterization of the SP+ cell line demonstrated the lack of thymic T-cell precursor markers, including absence of cell surface expression of CD1, intracellular thymic terminal deoxynucleotidyl transferase (TdT) enzyme, as well as message expression for V(D)J recombinase activating gene-1 (RAG-1). Furthermore, the SP+ cell did express the message for the CD3 delta chain. Taken together, these data suggest that the SP+ cell line resulted from HTLV-I infection of a mature CD4(+)/CDB+ lymphocyte. This cell line can be potentially useful as a model, both for regulation of cellular functions by HTLV-I and for immunologic functions of mature dual CD4/CD8 positive T-cells.
引用
收藏
页码:621 / 628
页数:8
相关论文
共 28 条
[1]  
AKAGI T, 1985, ACTA MED OKAYAMA, V39, P155
[2]  
BLUE ML, 1985, J IMMUNOL, V134, P2281
[3]  
CHEN ISY, 1993, P NATL ACAD SCI USA, V80, P7006
[4]   CHARACTERIZATION OF HUMAN T-LYMPHOTROPIC VIRUS TYPE I-INFECTED AND II-INFECTED T-CELL LINES - ANTIGENIC, PHENOTYPIC, AND GENOTYPIC ANALYSIS [J].
DEZZUTTI, CS ;
RUDOLPH, DL ;
ROBERTS, CR ;
LAL, RB .
VIRUS RESEARCH, 1993, 29 (01) :59-70
[5]   ONTOGENY OF T-CELL PRECURSORS - A MODEL FOR THE INITIAL-STAGES OF HUMAN T-CELL DEVELOPMENT [J].
HAYNES, BF ;
DENNING, SM ;
SINGER, KH ;
KURTZBERG, J .
IMMUNOLOGY TODAY, 1989, 10 (03) :87-91
[6]   SENSITIVE AND SPECIFIC POLYMERASE CHAIN-REACTION ASSAYS FOR DIAGNOSIS OF HUMAN T-CELL LYMPHOTROPIC VIRUS TYPE-I (HTLV-I) AND HTLV-II INFECTIONS IN HTLV-I/II-SEROPOSITIVE INDIVIDUALS [J].
HENEINE, W ;
KHABBAZ, RF ;
LAL, RB ;
KAPLAN, JE .
JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (06) :1605-1607
[7]  
HOLLSBERG P, 1993, NEW ENGL J MED, V328, P1173, DOI 10.1056/NEJM199304223281608
[8]  
KAY NE, 1990, BLOOD, V75, P2024
[9]   INFECTION OF A LABORATORY WORKER WITH SIMIAN IMMUNODEFICIENCY VIRUS [J].
KHABBAZ, RF ;
HENEINE, W ;
GEORGE, JR ;
PAREKH, B ;
ROWE, T ;
WOODS, T ;
SWITZER, WM ;
MCCLURE, HM ;
MURPHEYCORB, M ;
FOLKS, TM .
NEW ENGLAND JOURNAL OF MEDICINE, 1994, 330 (03) :172-177
[10]   SIMULTANEOUS EXPRESSION OF T-CELL AND MYELOID CELL PHENOTYPES IN 8 NEWLY ESTABLISHED HTLV-I-POSITIVE T-CELL LINES [J].
KOIZUMI, S ;
SUGIURA, M ;
ZHANG, XK ;
YAMASHIRO, K ;
IWANAGA, M ;
IMAI, S ;
OSATO, T .
JAPANESE JOURNAL OF CANCER RESEARCH, 1992, 83 (09) :929-932