PRESENCE OF NUCLEAR ANDROGEN RECEPTOR-LIKE IMMUNOREACTIVITY IN NEUROKININ B-CONTAINING NEURONS OF THE HYPOTHALAMIC ARCUATE NUCLEUS OF THE ADULT MALE-RAT

Dual label immunofluorescence was used in the brain of normal, non colchicine-treated, adult male rats in order to characterize the neurons possessing the nuclear androgen receptor in the hypothalamic arcuate nucleus, The affinity purified PG21 rabbit serum to the rat androgen receptor (AR) was used in conjunction with a polyclonal guinea pig antiserum to peptide 2, a sequence within the precursor to neurokinin B (NKB). All NKB-containing neurons were also immunoreactive for the AR, and made up a large proportion (about 60%) of the arcuate cells with detectable and specific AR immunoreactivity.