DIFFERENTIAL-EFFECTS OF SPERMINE AND ITS ANALOGS ON THE STRUCTURES OF POLYNUCLEOTIDES COMPLEXED WITH ETHIDIUM-BROMIDE

被引:33
作者
DELCROS, JG
STURKENBOOM, MCJM
BASU, HS
SHAFER, RH
SZOLLOSI, J
FEUERSTEIN, BG
MARTON, LJ
机构
[1] UNIV CALIF SAN FRANCISCO,SCH MED,DEPT NEUROL SURG,BRAIN TUMOUR RES CTR,SAN FRANCISCO,CA 94143
[2] UNIV CALIF SAN FRANCISCO,SCH MED,DEPT LAB MED,SAN FRANCISCO,CA 94143
[3] UNIV CALIF SAN FRANCISCO,SCH MED,DEPT PEDIAT,SAN FRANCISCO,CA 94143
[4] UNIV CALIF SAN FRANCISCO,SCH PHARM,DEPT PHARMACEUT CHEM,SAN FRANCISCO,CA 94143
关键词
D O I
10.1042/bj2910269
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The interactions of spermine and polyamine analogues with synthetic polynucleotides of various base sequences complexed with ethidium bromide (EB) were investigated using measurements of fluorescence intensity and steady-state fluorescence polarization. Spermine and polyamine analogues displaced some but not all of the EB bound to poly(dA-dT) . poly(dA-dT) or poly(dG-dC) . poly(dG-dC), suggesting that polyamines may stabilize these polynucleotides in a conformation with reduced affinity for EB. Modifications of the aliphatic backbone of spermine have pronounced effects on its ability to displace EB from poly(dA-dT) . poly(dA-dT) but not from poly(dG-dC) . poly(dG-dC). Spermine and some but not all of the polyamine analogues caused fluorescence depolarization when they interacted with the complex of EB and poly(dA-dT) . poly(dA-dT). Neither spermine nor any of the analogues, however, induced fluorescence depolarization in the complex of EB with poly(dG-dC) . poly(dG-dC) or poly(dA) . poly(dT). This suggests that spermine and some spermine analogues induce structural changes specific to alternating A-T sequences.
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页码:269 / 274
页数:6
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