DETECTION OF A CYTOTOXIC SUBSTANCE PRODUCED BY THE CYANOBACTERIUM MICROCYSTIS-AERUGINOSA STRAIN PCC-7806 - ISOLATION AND DIFFERENTIATION FROM THE PEPTIDE TOXIN MICROCYSTIN-LR BY CYTOTOXICITY ASSAYS

被引：7

作者：

HENNING, K ^{[1
]}

MEYER, H ^{[1
]}

KRAATZWADSACK, G ^{[1
]}

CREMER, J ^{[1
]}

机构：

[1] FED ARMED FORCES MED ACAD,INST MICROBIOL,MUNICH,GERMANY

来源：

CURRENT MICROBIOLOGY | 1992年 / 25卷 / 03期

关键词：

D O I：

10.1007/BF01571020

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Exposure of four permanent cell lines to crude aqueous extracts of the cyanobacterium Microcystis aeruginosa (strain PCC 7806) resulted in rounding and lysis of the cells within a few minutes. Cell damage was quantified by determination of lactate dehydrogenase (LDH) activity in the cell culture supernatants. By gel filtration, cation exchange chromatography and SDS-PAGE, the cytotoxic effects could be related to a defined substance with an apparent molecular weight of about 35 kDa, which was not heat resistant. The physicochemical properties very clearly mark off this compound from the peptide toxin microcystin-LR, which did not show any detectable cytotoxic effects.

引用

页码：129 / 134

页数：6

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