A SPECIES-SPECIFIC OLIGONUCLEOTIDE DNA PROBE FOR THE IDENTIFICATION OF MELOIDOGYNE-INCOGNITA

被引：10

作者：

CHACON, MR

PARKHOUSE, RME

ROBINSON, MP

BURROWS, PR

GARATE, T

机构：

[1] CTR NACL BIOTECNOL,C SERRANO 115,MADRID,SPAIN

[2] NATL INST MED RES,DIV IMMUNOL,LONDON NW7 1AA,ENGLAND

[3] AFRC,INST ARABLE CROPS RES,DEPT ENTOMOL & NEMATOL,HARPENDEN AL5 2JQ,HERTS,ENGLAND

来源：

PARASITOLOGY | 1991年 / 103卷

关键词：

MELOIDOGYNE; OLIGONUCLEOTIDE; DNA PROBE; DIAGNOSIS;

D O I：

10.1017/S003118200005959X

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A genomic library of Meloidogyne incognita Race 1 has been prepared in the bacteriophage-lambda-gt10 and screened for specific DNA sequences by hybridization with radio-isotope labelled total genomic DNA from a number of Meloidogyne species. One clone isolated (MR1#15), although not totally species specific, clearly showed preferential hybridization to M. incognita. Following subcloning and sequencing of the 255 bp insert, four stretches of the sequence corresponding to oligonucleotides of approximately equal length (approximately 60 bp) were synthesized and examined for specificity. One of them, MR1#15.2, showed the necessary specificity to be used as a diagnostic tool.

引用

页码：315 / 319

页数：5

共 26 条

[1] MOLECULAR APPROACHES TO DNA DIAGNOSIS [J].