HUMAN TYPE-2 ANGIOTENSIN-II RECEPTOR GENE - CLONED, MAPPED TO THE X-CHROMOSOME, AND ITS MESSENGER-RNA IS EXPRESSED IN THE HUMAN LUNG

The human type 2 angiotensin II receptor (AT(2)R) gene was isolated from a human genomic DNA library of adult female leukocytes. The putative coding region of the human AT(2)R gene is intronless. The primary structure of the human AT(2)R deduced from the nucleotide sequence of this putative coding region contains 363 amino acid residues and is highly homologous to the sequences of the rat and mouse AT(2)R. The human AT(2)R gene was mapped to the X chromosome using chromosomal assignment analysis with a human/rodent somatic cell hybrid panel. Northern blot analysis demonstrated a 3.0 kb mRNA in the human fetal lung and kidney and the human adult lung. These data and probes should enhance research in human AT(2)R biology. (C) 1994 Academic Press, Inc.