ALLELIC LOSSES IN MUTATIONS AT THE APRT LOCUS OF HUMAN LYMPHOBLASTOID-CELLS

被引:26
作者
FUJIMORI, A [1 ]
TACHIBANA, A [1 ]
TATSUMI, K [1 ]
机构
[1] KYOTO UNIV,FAC MED,DEPT MOLEC ONCOL,YOSHIDAKONOE CHO,SAKYO KU,KYOTO 606,JAPAN
来源
MUTATION RESEARCH | 1992年 / 269卷 / 01期
基金
日本科学技术振兴机构;
关键词
APRT GENE; ALLELIC LOSS; SPONTANEOUS MUTATION; GAMMA-RAY-INDUCED MUTATION; CARCINOGENESIS;
D O I
10.1016/0027-5107(92)90160-4
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
We analyzed the nature of mutations at the autosomal locus coding for adenine phosphoribosyltransferase (aprt) in human cells to elucidate the process(es) governing mutagenesis at autosomal loci. A human lymphoblastoid cell line, WR10, was found to be heterozygous for mutated allele at the aprt locus, and was used for mutation analyses. By the use of a restriction fragment length polymorphism associated with the aprt locus in WR10 cells, the molecular characteristics of mutations arising spontaneously or induced by gamma-rays were investigated. Eighty-five percent (22/26) of the spontaneous mutant clones and 93% (64/69) of the gamma-ray-induced mutant clones resulted from loss of one of the two aprt alleles. Determination of the dosage of aprt genes in those mutants with allelic losses revealed that approximately half of them retained two copies of the mutated allele. These data suggest that the mutational events leading to APRT deficiency are analogous to those reported for tumor suppressor genes in malignancies.
引用
收藏
页码:55 / 62
页数:8
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