A YEAST GENE FOR TREHALOSE-6-PHOSPHATE SYNTHASE AND ITS COMPLEMENTATION OF AN ESCHERICHIA-COLI OTSA MUTANT

被引：27

作者：

MCDOUGALL, J

KAASEN, I

STROM, AR

机构：

[1] The Norwegian College, Fishery Science The University of Tromsø, Tromsø

来源：

FEMS MICROBIOLOGY LETTERS | 1993年 / 107卷 / 01期

关键词：

TREHALOSE-6-PHOSPHATE SYNTHASE; SACCHAROMYCES-CEREVISIAE; OTSA GENE; TPS1; GGS1;

D O I：

10.1111/j.1574-6968.1993.tb05998.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A Saccharomyces cerevisiae gene for trehalose-6-phosphate synthase (TPS1) was sequenced. The gene appeared to code for a protein of 495 amino acid residues, giving the protein a molecular mass of 56 kDa. The TPS1 gene was able to restore both osmotolerance and trehalose accumulation during salt stress in an Escherichia coli strain mutated in the otsA gene encoding trehalose-6-phosphate synthase. Complementation studies with E. coli galU mutants showed that-the TPS1-encoded trehalose-6-phosphate synthase is UDP-glucose-dependent. Sequence analysis and data base searches showed that TPS1 is allelic to GGS1, byp1, cif1 and fdp1. A possible gene for trehalose-6-phosphate synthase in Methanobacterium thermoautotrophicum was identified.

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页码：25 / 30

页数：6

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