PEPTIDE MIMETICS OF THYROTROPIN-RELEASING-HORMONE BASED ON A CYCLOHEXANE FRAMEWORK - DESIGN, SYNTHESIS, AND COGNITION-ENHANCING PROPERTIES

The design and synthesis of peptide mimetics of thyrotropin-releasing hormone (TRH) in which the peptide backbone is entirely replaced by a cyclohexane framework are described. The cis-1,3,5-trisubstituted ring was expected to permit key pharmacophoric groups to adopt conformations consistent with proposed bioactive conformations of the peptide. Compounds were synthesized by a stereoselective synthesis starting from L-glutamic acid. In a behavioral model of cognition in which TRH is active, the mimetics are potent, active compounds, exhibiting oral activity. One analog (26, (1S,3R,5(2S),5S)-5-[[5-oxo-1-(phenylmethyl)-2-pyrrolidinyl]- methyl]-5-[(1H-imidazol-5-yl)methyl]cyclohexaneacetamide) was radiolabeled for binding studies and evaluated in other binding assays and pharmacological tests. Competition binding of 26 vs [H-3]MeTRH to rat brain slices suggests a two-site model for ligand binding with IC50's of 1 mu M and 3 mM. Direct binding of [H-3]-26 shows a biphasic curve with IC50's Of 80 and 49 mu M, respectively. Further studies would be needed to establish a link between the novel binding site(s) and the behavioral activity of 26 and TRH analogs.