DIFFERENTIAL LOCALIZATION OF HEME OXYGENASE AND NADPH-DIAPHORASE IN SPINAL-CORD NEURONS

被引：38

作者：

DWYER, BE

NISHIMURA, RN

LU, SY

机构：

[1] DEPT VET AFFAIRS MED CTR,IN VITRO REMYELINAT LAB,SEPULVEDA,CA 91343

[2] UNIV CALIF LOS ANGELES,SCH MED,DEPT NEUROL,LOS ANGELES,CA 90024

[3] UNIV CALIF LOS ANGELES,SCH MED,BRAIN RES INST,LOS ANGELES,CA 90024

来源：

NEUROREPORT | 1995年 / 6卷 / 07期

关键词：

HEME OXYGENASE; SPINAL CORD; NEURON; MOTOR NEURON; NITRIC OXIDE SYNTHASE; NADPH-DIAPHORASE; CARBON MONOXIDE; HEAT SHOCK PROTEIN; STRESS PROTEIN;

D O I：

10.1097/00001756-199505090-00006

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

WESTERN blot analysis using several antibodies showed that rat spinal cord contained abundant immunostainable heme oxygenase-2 (HO-2) and barely detectable levels of heme oxygenase-1 (HO-1). Anti-HO-2 antibody stained large anterior horn motoneurones and numerous smaller neurons throughout spinal cord gray matter including the dorsal root entry zone. HO-2(+) astrocytes were not evident in gray matter although their presence cannot be ruled out. The distribution of HO-2(+) neurons was compared with the distribution of cells containing NADPH-diaphorase (NADPH-d) activity, a marker for nitric oxide synthase. NADPH-d activity was restricted to far fewer neurons, many of which were close to the central canal and dorsal root entry zone.

引用

页码：973 / 976

页数：4