THE VIRULENCE-ASSOCIATED CHRYSOBACTIN IRON UPTAKE SYSTEM OF ERWINIA-CHRYSANTHEMI-3937 INVOLVES AN OPERON ENCODING TRANSPORT AND BIOSYNTHETIC FUNCTIONS

被引：35

作者：

FRANZA, T ^{[1
]}

EXPERT, D ^{[1
]}

机构：

[1] INRA,PATHOL VEGETALE LAB,16 RUE CLAUDE BERNARD,F-75231 PARIS 05,FRANCE

来源：

JOURNAL OF BACTERIOLOGY | 1991年 / 173卷 / 21期

关键词：

D O I：

10.1128/jb.173.21.6874-6881.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The iron assimilation system of Erwinia chrysanthemi 3937 is mediated by the catechol-type siderophore chrysobactin and the outer membrane transport protein Fct. We generated a variety of subclones in high- and low-copy-number vectors from a wild-type recombinant cosmid shown previously to carry the gene cluster fct-cbsA, cbsB, cbsC, cbsE encoding chrysobactin transport and biosynthetic functions, respectively. We studied their expression in Escherichia coli enterobactin-deficient entA, entB, entC, and entE mutants. This provided evidence that the fct and cbs genes are regrouped within a single genetic unit of ca. 8 kb in the following order: fct, cbsC, cbsE, cbsB, and cbsA. The gene boundaries were determined, and the various recombinant plasmids were expressed in Escherichia coli minicells: CbsA and CbsC enzymatic activities were clearly identified as polypeptides with apparent molecular masses of 32,000 and 38,000, respectively.

引用

页码：6874 / 6881

页数：8

共 33 条

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