SELECTION OF NODULATION AND MYCORRHIZAL MUTANTS IN THE MODEL-PLANT MEDICAGO-TRUNCATULA (GAERTN) AFTER GAMMA-RAY MUTAGENESIS

被引：171

作者：

SAGAN, M ^{[1
]}

MORANDI, D ^{[1
]}

TARENGHI, E ^{[1
]}

DUC, G ^{[1
]}

机构：

[1] INRA,PHYTOPARASITOL LAB,GENET & AMELIORAT PLANTES STN,F-21034 DIJON,FRANCE

来源：

PLANT SCIENCE | 1995年 / 111卷 / 01期

关键词：

MEDICAGO TRUNCATULA; SYMBIOSIS; GAMMA-RAY MUTAGENESIS; NODULATION; NITROGEN FIXATION; ENDOMYCORRHIZA;

D O I：

10.1016/0168-9452(95)04229-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Seeds of Medicago truncatula cv. Jemalong line J5 were treated by gamma-rays to create mutations affecting Rhizobium symbiosis. Putative mutants were selected in M(2) and true mutant phenotypes were confirmed in M(3), M(4), M(5) and M(6) generations. Fixed nodulation mutant lines were then screened for their endomycorrhizal characters. Eighteen mutant lines are now available. They consist of 2 non-nodulating and non-mycorrhizal (Nod(-), Myc(-)), 4 low-nodulating and mycorrhizal (Nod(+/-), Myc(+)), 9 nodulating, non-fixing and mycorrhizal (Nod(+)Fix(-), Myc(+)) and 3 supernodulating, nitrate tolerant nodulation and mycorrhizal (Nod(++)Nts, Myc(+)) mutant lines.

引用

页码：63 / 71

页数：9

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