TYPE-I INOSINE MONOPHOSPHATE DEHYDROGENASE - EVIDENCE FOR A SINGLE FUNCTIONAL GENE IN MAMMALIAN-SPECIES

被引：10

作者：

DAYTON, JS

MITCHELL, BS

机构：

[1] UNIV MICHIGAN, DEPT PHARMACOL, ANN ARBOR, MI 48109 USA

[2] UNIV N CAROLINA, DEPT INTERNAL MED, CHAPEL HILL, NC 27599 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 195卷 / 02期

关键词：

D O I：

10.1006/bbrc.1993.2129

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human inosine monophosphate dehydrogenase activity is the result of the expression of two independent but closely related genes, termed IMPDH type I and type II. We have documented the presence of multiple, processed pseudogenes of type I IMPDH in human and Rhesus monkey genomic DNA, as well as a single functional gene encoding low levels of type I mRNA in human brain, heart, kidney and placenta. Single copy genes for each IMPDH isoenzyme were also found in rat, mouse, dog, cos, and chicken DNA and distinct mRNA species for type I and type II were identified by Northern blots in mouse and hamster RNA. Northern blot analysis of chicken RNA revealed a single mRNA species that hybridized to human IMPDH type I and II probes. These data document the high degree of evolutionary conservation of these two genes among mammals. © 1993 Academic Press, Inc.

引用

页码：897 / 901

页数：5

共 24 条

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