PROTEIN-SYNTHESIS-DEPENDENT INDUCTION OF ANNEXIN-I BY GLUCOCORTICOID

被引：38

作者：

WONG, WT ^{[1
]}

FROST, SC ^{[1
]}

NICK, HS ^{[1
]}

机构：

[1] UNIV FLORIDA,COLL MED,DEPT BIOCHEM & MOLEC BIOL,GAINESVILLE,FL 32610

来源：

BIOCHEMICAL JOURNAL | 1991年 / 275卷

关键词：

D O I：

10.1042/bj2750313

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We demonstrate that annexin I/lipocortin I (lipo I) gene expression is regulated by dexamethasone (DEX) in mouse 3T3-L1 fibroblasts and LA-4 lung epithelial cells. We have characterized this induction further in 3T3-L1 fibroblasts. At 24 h after addition of DEX, the levels of lipo I mRNA and protein increased 5-fold and 1.5-fold respectively. Time-course experiments revealed that the induction was delayed by 2-4 h after DEX addition. Half-maximal induction of both lipo I mRNA and protein was achieved with 10 nM-DEX. Both actinomycin D and cycloheximide blocked the DEX effect on lipo I mRNA expression. These results indicate that the induction of lipo I by DEX has a transcriptional component and requires protein synthesis de novo.

引用

页码：313 / 319

页数：7

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