GLUTATHIONE AND CYSTEINE MEASUREMENT IN BIOLOGICAL SAMPLES BY HPLC WITH A GLASSY-CARBON WORKING DETECTOR

被引：52

作者：

CARVALHO, FD

REMIAO, F

VALE, P

TIMBRELL, JA

BASTOS, ML

FERREIRA, MA

机构：

[1] UNIV PORTO, FAC PHARM, BROMATOL LAB, P-4000 OPORTO, PORTUGAL

[2] UNIV LONDON SCH PHARM, DEPT TOXICOL, LONDON, ENGLAND

来源：

BIOMEDICAL CHROMATOGRAPHY | 1994年 / 8卷 / 03期

关键词：

D O I：

10.1002/bmc.1130080308

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

An HPLC method using a glassy carbon working detector for measuring cysteine and reduced glutathione (GSH) in biological samples was developed. Oxidized glutathione (GSSG) was also measured after reduction with glutathione reductase. This study was conducted in human plasma. Cysteine and GSH standard curves were linear in the physiological range, presenting detection limits of 22.0 pmol and 6.0 pmol respectively. Plasmatic results found were 43.92 +/- 4.15, 4.50 +/- 0.65, and 0.19 +/- 0.12 mM (means +/- SE), for cysteine, GSH and GSSG, respectively. Peak specificity for cysteine and reduced glutathione was certified by their disappearance after treatment with N-ethylmaleimide.

引用

页码：134 / 136

页数：3

共 23 条

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[2]

DIVERGENT EFFECTS OF INTRAVENOUS GSH AND CYSTEINE ON RENAL AND HEPATIC GSH [J].