STAINING OF HUMAN TELOMERES WITH PRIMED IN-SITU LABELING (PRINS)

被引：67

作者：

THERKELSEN, AJ

NIELSEN, A

KOCH, J

HINDKJAER, J

KOLVRAA, S

机构：

[1] Institute of Human Genetics, The Bartholin Building, Aarhus University

来源：

CYTOGENETICS AND CELL GENETICS | 1995年 / 68卷 / 1-2期

关键词：

D O I：

10.1159/000133903

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

As described, the PRINS method is a very rapid and reliable way of staining human telomeres. To obtain the maximum frequency of stained telomeres, the primer (CCCTAA)(7) should be used, although the average frequency never quite reaches 100%. The frequency is strongly dependent on the age of the individual, being significantly higher in children and newborns than in adults. A difference between the (CCCTAA)(7) primer and the complementary primer is demonstrated and a possible explanation is proposed, namely, that gaps in the C-rich strand cause chain elongation termination after the addition of only one dTTP molecule.

引用

页码：115 / 118

页数：4

共 11 条

[1] NEW SYSTEM OF CHROMOSOME BANDING - T-BANDS
DUTRILLAUX, B
[J]. CHROMOSOMA, 1973, 41 (04) : 395 - 402
[2] OLIGONUCLEOTIDE-PRIMED INSITU DNA-SYNTHESIS (PRINS) - A METHOD FOR CHROMOSOME MAPPING, BANDING, AND INVESTIGATION OF SEQUENCE ORGANIZATION
GOSDEN, J
HANRATTY, D
STARLING, J
FANTES, J
MITCHELL, A
PORTEOUS, D
[J]. CYTOGENETICS AND CELL GENETICS, 1991, 57 (2-3): : 100 - 104
[3] TELOMERES SHORTEN DURING AGING OF HUMAN FIBROBLASTS
HARLEY, CB
FUTCHER, AB
GREIDER, CW
[J]. NATURE, 1990, 345 (6274) : 458 - 460
[4] TELOMERE REDUCTION IN HUMAN COLORECTAL-CARCINOMA AND WITH AGING
HASTIE, ND
DEMPSTER, M
DUNLOP, MG
THOMPSON, AM
GREEN, DK
ALLSHIRE, RC
[J]. NATURE, 1990, 346 (6287) : 866 - 868
[5] THE TELOMERE IN CANCER - ALLS NOT WELL THAT DOESNT END WELL
HECHT, F
HECHT, BK
[J]. CANCER GENETICS AND CYTOGENETICS, 1991, 54 (02) : 245 - 246
[6] OLIGONUCLEOTIDE-PRIMING METHODS FOR THE CHROMOSOME-SPECIFIC LABELING OF ALPHA-SATELLITE DNA INSITU
KOCH, JE
KOLVRAA, S
PETERSEN, KB
GREGERSEN, N
BOLUND, L
[J]. CHROMOSOMA, 1989, 98 (04) : 259 - 265
[7] TELOMERE CAPTURE STABILIZES CHROMOSOME BREAKAGE
MELTZER, PS
GUAN, XY
TRENT, JM
[J]. NATURE GENETICS, 1993, 4 (03) : 252 - 255
[8] A HIGHLY CONSERVED REPETITIVE DNA-SEQUENCE, (TTAGGG)N, PRESENT AT THE TELOMERES OF HUMAN-CHROMOSOMES
MOYZIS, RK
BUCKINGHAM, JM
CRAM, LS
DANI, M
DEAVEN, LL
JONES, MD
MEYNE, J
RATLIFF, RL
WU, JR
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (18) : 6622 - 6626
[9] TELOMERE POSITIONAL EFFECTS AND THE REGULATION OF CELLULAR SENESCENCE
WRIGHT, WE
SHAY, JW
[J]. TRENDS IN GENETICS, 1992, 8 (06) : 193 - 197
[10] REPEATED HEXANUCLEOTIDE C-C-C-C-A-A IS PRESENT NEAR FREE ENDS OF MACRONUCLEAR DNA OF TETRAHYMENA
YAO, MC
YAO, CH
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (12): : 7436 - 7439

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