DETERMINATION OF HYDROXYMETHYLFURFURAL IN BABY CEREALS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：40

作者：

GUERRAHERNANDEZ, E

GARCIAVILLANOVA, B

MONTILLAGOMEZ, J

机构：

[1] Departamento de Nutritión y Bromatologia, Facultdad de Farrnacia, Universidad de Granada, 18012, Granada

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1992年 / 15卷 / 14期

关键词：

D O I：

10.1080/10826079208017201

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A liquid chromatography method for the detection and quantification of 5-hydroxymethyl-2-furaldehyde (HMF) in baby cereals is described. HMF was obtained by centrifugation and the supernatant was clarified by the addition of Carrez solution. The mixture was centrifuged for 5 min at 5,000 rpm. The samples were analysed on a reverse-phase C,8 column and ultraviolet detector (284 nm) in isocratic elution with 95:5 water-acetonitrile. Average recovery of HMF by the standard addition method was 96.9%. The variation coefficients for three levels of concentration, (3.24, 22.82 and 65.50 ppm) were 2.40, 2.14 and 1.09% respectively. Limit of detection was less-than-or-equal-to 0.09 ppm. This method was applied by determination of HMF in commercial baby cereals.

引用

页码：2551 / 2559

页数：9